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KMID : 0828520060100040278
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Journal of the Korean Geriatrics Society
2006 Volume.10 No. 4 p.278 ~ p.284
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Subcellular Distribution of Microtubule in Artificially Induced Senescent PC12 Cells
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Lee Eun-Ju
Oh Chang-Seok
Shin Dong-Hoon
Lee Young-Soo
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Abstract
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Background: Since recent reports showed the possibility that cytoskeletal proteins, which were known to be exclusively within the cytoplasm, might play a role in the re-distribution of the intranuclear chromatin in a certain type of the cells under specific circumstances, we tried to show a change in the intracellular distribution of microtubule protein in artificially induced senescent PC12 pheochromocytoma cells.
Methods: PC12 pheochromocytoma cells (ATCC CRL-1721) were grown in the culture media including 1 μM 3’-Azido-3’-deoxythy-midine (AZT, Sigma-Aldrich, USA). The senescence of the cells was confirmed by senescence detection kit (Calbiochem, San Diego, CA). Immunocytochemical study was also performed in the cells treated with AZT during 0, 75 and 153 days.
Results: β-tubuline was not observed in the cells not treated with AZT. The same protein was localized within the nuclei in the senescent cells treated with AZT during 153 days.
Conclusion: Microtubule might be involved in some crucial roles in the redistribution of chromatin within the nuclei of the senescent cells.
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KEYWORD
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Microtubule, PC12, Cellular senescence, AZT, Tubuline, MTOC
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